cell line kasumi-1 Search Results


90
BioResource International Inc aml cell line kasumi-1
GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and <t>AML</t> <t>cell</t> lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Aml Cell Line Kasumi 1, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
JCRB Cell Bank human acute myeloblastic leukemia cell line kasumi-1
GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and <t>AML</t> <t>cell</t> lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Human Acute Myeloblastic Leukemia Cell Line Kasumi 1, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human acute myeloblastic leukemia cell line kasumi-1 - by Bioz Stars, 2026-09
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90
National Centre for Cell Science human myeloid leukemic kasumi-1 cell line
GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and <t>AML</t> <t>cell</t> lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Human Myeloid Leukemic Kasumi 1 Cell Line, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human myeloid leukemic kasumi-1 cell line - by Bioz Stars, 2026-09
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90
BioResource International Inc kasumi-1 cell line
GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and <t>AML</t> <t>cell</t> lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Kasumi 1 Cell Line, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+kasumi-1/cell+line+kasumi+1+ckit+/pmc06372575-204-0-12
Average 90 stars, based on 1 article reviews
kasumi-1 cell line - by Bioz Stars, 2026-09
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90
ChemPartner aml cell line-derived xenograft (cdx) model kasumi-1
GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and <t>AML</t> <t>cell</t> lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Aml Cell Line Derived Xenograft (Cdx) Model Kasumi 1, supplied by ChemPartner, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
aml cell line-derived xenograft (cdx) model kasumi-1 - by Bioz Stars, 2026-09
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90
LGC Promochem kasumi-1 cell line
GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and <t>AML</t> <t>cell</t> lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Kasumi 1 Cell Line, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+kasumi-1/kasumi+1+cell+line/pm18840553-33-0-19
Average 90 stars, based on 1 article reviews
kasumi-1 cell line - by Bioz Stars, 2026-09
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N/A
The KASUMI-1 (NFkB) Luciferase cell line is transformed from KASUMI-1 cell, expressing the firefly luciferase gene. The cell constitutively express Luciferase.
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N/A
The KASUMI-1 (STAT3) Luciferase cell line is transformed from KASUMI-1 cell, expressing the firefly luciferase gene. The cell constitutively express Luciferase.
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N/A
The KASUMI-1 (SMAD/TGFbeta) Luciferase cell line is transformed from KASUMI-1 cell, expressing the firefly luciferase gene. The cell constitutively express Luciferase.
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Image Search Results


GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.

Journal: Cancer Biomarkers

Article Title: Glutaminase 1 plays critical roles in myelodysplastic syndrome and acute myeloid leukemia cells

doi: 10.3233/CBM-230454

Figure Lengend Snippet: GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.

Article Snippet: The MDS cell line SKM-1 (monoblastic leukemia following MDS) and the AML cell lines MOLM-14 (acute monocytic leukemia: AML-M5a) and Kasumi-1 (8;21 chromosome translocation) were obtained from the Japan Research Bioresource Cell Bank (Ibaraki, Osaka, Japan).

Techniques: Gene Expression, Activity Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Control, Incubation, Cell Counting, Cell Viability Assay, Lactate Dehydrogenase Assay, Caspase-Glo Assay

Activity of GLS1 inhibitors in MDS and AML cells. (A, B) MDS and AML cell lines were cultured with the indicated concentrations of CB839 or IPN60090 for 72 h under hypoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (C, D, E, F) MDS and AML cell lines were cultured with the indicated concentrations of CB838 or IPN60090 for 24 h. The total amount of NADPH or NADP + was analyzed by using NADP/NADPH Assay Kit-WST. (G) SKM-1 cells were incubated with the indicated concentration of CB839 or IPN60090 for 72 h. Intracellular ATP levels were determined using the “Cell” ATP Assay Reagent Ver. 2 Kit. *** p< 0.001, **** p< 0.0001 were compared to control. ns, not significant. (H) SKM-1 cells were incubated with the indicated concentration of CB839 for 24 h. The extracellular OCR and the extracellular acidification were analyzed.

Journal: Cancer Biomarkers

Article Title: Glutaminase 1 plays critical roles in myelodysplastic syndrome and acute myeloid leukemia cells

doi: 10.3233/CBM-230454

Figure Lengend Snippet: Activity of GLS1 inhibitors in MDS and AML cells. (A, B) MDS and AML cell lines were cultured with the indicated concentrations of CB839 or IPN60090 for 72 h under hypoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (C, D, E, F) MDS and AML cell lines were cultured with the indicated concentrations of CB838 or IPN60090 for 24 h. The total amount of NADPH or NADP + was analyzed by using NADP/NADPH Assay Kit-WST. (G) SKM-1 cells were incubated with the indicated concentration of CB839 or IPN60090 for 72 h. Intracellular ATP levels were determined using the “Cell” ATP Assay Reagent Ver. 2 Kit. *** p< 0.001, **** p< 0.0001 were compared to control. ns, not significant. (H) SKM-1 cells were incubated with the indicated concentration of CB839 for 24 h. The extracellular OCR and the extracellular acidification were analyzed.

Article Snippet: The MDS cell line SKM-1 (monoblastic leukemia following MDS) and the AML cell lines MOLM-14 (acute monocytic leukemia: AML-M5a) and Kasumi-1 (8;21 chromosome translocation) were obtained from the Japan Research Bioresource Cell Bank (Ibaraki, Osaka, Japan).

Techniques: Activity Assay, Cell Culture, Cell Viability Assay, Cell Counting, Incubation, Concentration Assay, ATP Assay, Control