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The KASUMI-1 (NFkB) Luciferase cell line is transformed from KASUMI-1 cell, expressing the firefly luciferase gene. The cell constitutively express Luciferase.
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Image Search Results
Journal: Cancer Biomarkers
Article Title: Glutaminase 1 plays critical roles in myelodysplastic syndrome and acute myeloid leukemia cells
doi: 10.3233/CBM-230454
Figure Lengend Snippet: GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.
Article Snippet: The MDS cell line SKM-1 (monoblastic leukemia following MDS) and the
Techniques: Gene Expression, Activity Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Control, Incubation, Cell Counting, Cell Viability Assay, Lactate Dehydrogenase Assay, Caspase-Glo Assay
Journal: Cancer Biomarkers
Article Title: Glutaminase 1 plays critical roles in myelodysplastic syndrome and acute myeloid leukemia cells
doi: 10.3233/CBM-230454
Figure Lengend Snippet: Activity of GLS1 inhibitors in MDS and AML cells. (A, B) MDS and AML cell lines were cultured with the indicated concentrations of CB839 or IPN60090 for 72 h under hypoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (C, D, E, F) MDS and AML cell lines were cultured with the indicated concentrations of CB838 or IPN60090 for 24 h. The total amount of NADPH or NADP + was analyzed by using NADP/NADPH Assay Kit-WST. (G) SKM-1 cells were incubated with the indicated concentration of CB839 or IPN60090 for 72 h. Intracellular ATP levels were determined using the “Cell” ATP Assay Reagent Ver. 2 Kit. *** p< 0.001, **** p< 0.0001 were compared to control. ns, not significant. (H) SKM-1 cells were incubated with the indicated concentration of CB839 for 24 h. The extracellular OCR and the extracellular acidification were analyzed.
Article Snippet: The MDS cell line SKM-1 (monoblastic leukemia following MDS) and the
Techniques: Activity Assay, Cell Culture, Cell Viability Assay, Cell Counting, Incubation, Concentration Assay, ATP Assay, Control